Journal: Heliyon

Article Title: The preventive effects of Saccharomyces boulardii against oxidative stress induced by lipopolysaccharide in rat brain

doi: 10.1016/j.heliyon.2024.e30426

Figure Lengend Snippet: Panels a–c show the effects of S. boulardii on the serum of IL-10, IL-6, and IL-8 respectively. Data are presented as mean ± SEM. *P < 0.05, and **P < 0.01, compared to control group. + P < 0.05, ++ P < 0.01, and ++++ P < 0.0001 compared to LPS group, #### P < 0.0001, compared to Sb group. N = 8. Control (Con), lipopolysaccharide (LPS), S. boulardii + lipopolysaccharide (Sb + LPS), S. boulardii (Sb).

Article Snippet: Following the collection of blood samples, the serum concentrations of IL-10 (DY522, Bio-Techne, USA), IL-6 (CSB-E04640r, Cusabio, USA), and IL-8 (abx576575, Abbexa, USA) were determined using ELISA (Enzyme-Linked Immunosorbent Assay) kits following the manufacturer's instructions.

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Respiratory syncytial virus G protein and G protein CX3C motif adversely affect CX3CR1+ T cell responses.

doi: 10.4049/jimmunol.176.3.1600

Figure Lengend Snippet: FIGURE 7. G protein affects the number of IL-4- and CX3CL1-expressing cells. Mice were i.n. challenged with 5 105 PFU of WT or dG virus. At days 6 p.i. (A and C) and 12 p.i. (B and D), spleen cells were stimu- lated in vitro with purified G protein (G, 1000 nM), WT virus (RSV, 106 PFU), Con A (25 mg/ml), cell-free un- infected VCL (106 PFU equivalent), or medium alone, and the number of IL-4 (A and B)- or CX3CL1 (C and D)-expressing cells (ELISPOTs) was determined. The data are presented as the mean SD of the number of ELISPOTs/106 spleen cells from three experiments us- ing three mice/infection/experiment.

Article Snippet: Spleen cells were isolated from naive, WT, or dG virus-infected mice at days 6 or 12 p.i., and cultured in triplicate at 1 106 cells/well in multiscreen polyvinylidene difluoride nitrocellulose plates (Millipore) coated with 4 mg/ml anti-mouse IL-4 Ab (clone 11B11; BD Pharmingen) or 4 mg/ml anti-mouse CX3CL1 (clone MAB581; R&D Systems).

Techniques: Expressing, Virus, In Vitro, Infection